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      Mouse TNF alpha PicoKine ELISA Kit

      2020/4/3 16:38:08 瀏覽:
      • 【品牌】: 博士德
      • 【產品貨號】: EK0527
      • 【價格】: 1200  元
      • 【規格】: 48T/96T
      • 【貨期】: 現貨
      • 在線訂購
      產品介紹
       產品說明
      【品牌】: 博士德
      【產品貨號】: EK0527
      【價格】: 1200  元
      【規格】: 48T/96T
      【貨期】: 現貨
      【儲存溫度】: -20

      Tumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.

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      準備試劑和標準品

      每孔添加100ul樣品和標準品,37℃反應90分鐘,不洗。

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      0.01M TBS洗滌3次。

      每孔添加100ulABC,37℃反應30分鐘。

      0.01M TBS洗滌5次。

      7 TMB37℃避光反應30分鐘以內。

      加入TMB終止液,讀數。


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