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          Mouse TNF alpha PicoKine ELISA Kit

          2020/4/3 16:38:08 瀏覽:
          • 【品牌】: 博士德
          • 【產品貨號】: EK0527
          • 【價格】: 1200  元
          • 【規格】: 48T/96T
          • 【貨期】: 現貨
          • 在線訂購
          產品介紹
           產品說明
          【品牌】: 博士德
          【產品貨號】: EK0527
          【價格】: 1200  元
          【規格】: 48T/96T
          【貨期】: 現貨
          【儲存溫度】: -20

          Tumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.

          典型操作程序:

          準備試劑和標準品

          每孔添加100ul樣品和標準品,37℃反應90分鐘,不洗。

          每孔添加100ul生物素標記抗體,37℃反應60分鐘。

          0.01M TBS洗滌3次。

          每孔添加100ulABC,37℃反應30分鐘。

          0.01M TBS洗滌5次。

          7 TMB37℃避光反應30分鐘以內。

          加入TMB終止液,讀數。


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